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产品简介:
本试剂盒利用竞争酶联免疫分析方法来测定细胞提取物或体外腺苷酸环化酶实验中的cGMP的水平。包被羊抗鼠多克隆抗体在酶标板上,细胞提取物或体外腺苷酸环化酶实验中的cGMP与固定数量的标记辣根过氧化物酶的cGMP竞争性的结合抗cGMP单克隆抗体,采用已知浓度的cGMP标准品来做标准曲线。检测450nm波长下的OD值,吸光度OD值与样品中cGMP的浓度呈反比关系。基于cGMP标准品所得曲线,通过测得的OD值可以计算出样品中cGMP的浓度,本试剂盒IC50(50% B/B0)约5 pmol/mL,检测限约0.1 pmol/mL。
组成成分 |
规格 |
保存 |
1. cGMP standard |
0.1ml |
-80℃ |
10000 pmol/mL cAMP |
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2. cGMP-HRP Conjugate |
0.2ml |
-80℃ |
1000x stock of HRP conjugated with cAMP |
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3. cGMP Antibody |
0.2ml |
-80℃ |
Anti-cAMP monoclonal antibody (1000X stock) |
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4. Assay Buffer(10x) |
30ml |
2-8℃ |
phosphate buffer with preservative |
||
5. NeutralizingReagent |
8ml |
2-8℃ |
tris buffered saline with preservative |
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6. Sample Diluent |
30ml |
2-8℃ |
0.1M HCl, only required for extraction of samples and diluting standard |
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7. TMB Substrate |
18ml |
2-8℃ |
3,3’,5,5’Tetramethylbenzidine |
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8. Stop Solution |
8ml |
2-8℃ |
2M sulphuric acid |
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9. Goat anti-Mouse IgG Coated microtiter plate |
96T |
2-8℃ |
12x8 well strips in a bag with desiccant |